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S phase index

S-phase index (SPI) is a measure of cell growth and viability. It is defined as the number of BrdU-incorporating cells relative to the volume of DNA staining determined from whole mount confocal analyses.

Only cells in the S Phase will incorporate BrdU into their DNA structure and so we can determine the length of the cell cycle using BrdU.

References

  • Murphy, Terence D. "Drosophila skpA, a component of SCF ubiquitin ligases, regulates centrosome duplication independently of cyclin E accumulation", Journal of Cell Science 116, 2321-2332 (2003).
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