S-(hydroxymethyl)glutathione dehydrogenase

In enzymology, a S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) is an enzyme that catalyzes the chemical reaction

S-(hydroxymethyl)glutathione + NAD(P)+ S-formylglutathione + NAD(P)H + H⁺

The 3 substrates of this enzyme are S-(hydroxymethyl)glutathione, NAD⁺, and NADP⁺, whereas its 4 products are S-formylglutathione, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is S-(hydroxymethyl)glutathione:NAD+ oxidoreductase. Other names in common use include NAD-linked formaldehyde dehydrogenase (incorrect), formaldehyde dehydrogenase (incorrect), formic dehydrogenase (incorrect), class III alcohol dehydrogenase, ADH3, &chi, -ADH, FDH (incorrect), formaldehyde dehydrogenase (glutathione) (incorrect), GS-FDH (incorrect), glutathione-dependent formaldehyde dehydrogenase (incorrect), NAD-dependent formaldehyde dehydrogenase, GD-FALDH, and NAD- and glutathione-dependent formaldehyde dehydrogenase. This enzyme participates in methane metabolism.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2FZE and 2FZW.

References

IUBMB entry for 1.1.1.284
BRENDA references for 1.1.1.284 (Recommended.)
PubMed references for 1.1.1.284
PubMed Central references for 1.1.1.284
Google Scholar references for 1.1.1.284
Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 7, Academic Press, New York, 1963, p. 203-221.
Rose ZB and Racker E (1966). "Formaldehyde dehydrogenase". Methods Enzymol. 9: 357–360.
Liu L, Hausladen A, Zeng M, Que L, Heitman J, Stamler JS (2001). "A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans". Nature. 410: 490–4. doi:10.1038/35068596. PMID 11260719.
Sanghani PC, Stone CL, Ray BD, Pindel EV, Hurley TD, Bosron WF (2000). "Kinetic mechanism of human glutathione-dependent formaldehyde dehydrogenase". Biochemistry. 39: 10720–9. doi:10.1021/bi9929711. PMID 10978156.
Probst C, Grotz M, Krettek C, Pape HC (2005). "Impact of hypothermia on the immunologic response after trauma and elective surgery". Surg. Technol. Int. 14: 41–50. PMID 16525953.
Stouthamer AH, Harms N (1995). "Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth". J. Bacteriol. 177: 247–51. PMID 7798140.
Barber RD, Rott MA, Donohue TJ (1996). "Characterization of a glutathione-dependent formaldehyde dehydrogenase from Rhodobacter sphaeroides". J. Bacteriol. 178: 1386–93. PMID 8631716.