L-lysine 6-monooxygenase (NADPH)

In enzymology, a L-lysine 6-monooxygenase (NADPH) (EC 1.14.13.59) is an enzyme that catalyzes the chemical reaction

L-lysine + NADPH + H⁺ + O₂ N₆-hydroxy-L-lysine + NADP⁺ + H₂O

The 4 substrates of this enzyme are L-lysine, NADPH, H⁺, and O₂, whereas its 3 products are N6-hydroxy-L-lysine, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating). This enzyme is also called lysine N6-hydroxylase. This enzyme participates in lysine degradation.

References

IUBMB entry for 1.14.13.59
BRENDA references for 1.14.13.59 (Recommended.)
PubMed references for 1.14.13.59
PubMed Central references for 1.14.13.59
Google Scholar references for 1.14.13.59
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Marrone L, Siemann S, Beecroft M and Viswanatha T (1996). "Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism". Bioorg. Chem. 24: 401–406. doi:10.1006/bioo.1996.0034.
Goh CJ, Szczepan EW, Menhart N, Viswanatha T (1989). "Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1". Biochim. Biophys. Acta. 990: 240–5. PMID 2493814.