2,5-didehydrogluconate reductase

In enzymology, a 2,5-didehydrogluconate reductase (EC 1.1.1.274) is an enzyme that catalyzes the chemical reaction

2-dehydro-D-gluconate + NADP⁺ 2,5-didehydro-D-gluconate + NADPH + H⁺

Thus, the two substrates of this enzyme are 2-dehydro-D-gluconate and NADP⁺, whereas its 3 products are 2,5-didehydro-D-gluconate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-dehydro-D-gluconate:NADP+ 2-oxidoreductase. Other names in common use include 2,5-diketo-D-gluconate reductase, and YqhE reductase.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1VP5.

References

IUBMB entry for 1.1.1.274
BRENDA references for 1.1.1.274 (Recommended.)
PubMed references for 1.1.1.274
PubMed Central references for 1.1.1.274
Google Scholar references for 1.1.1.274
Yum DY, Lee BY, Pan JG (1999). "Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli". Appl. Environ. Microbiol. 65: 3341–6. PMID 10427017.
Yum DY, Lee BY, Hahm DH, Pan JG (1998). "The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase". J. Bacteriol. 180: 5984–8. PMID 9811658.
Habrych M, Rodriguez S, Stewart JD (2002). "Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE". Biotechnol. Prog. 18: 257–61. doi:10.1021/bp0101841. PMID 11934293.