1-pyrroline-4-hydroxy-2-carboxylate deaminase

In enzymology, a 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22) is an enzyme that catalyzes the chemical reaction

1-pyrroline-4-hydroxy-2-carboxylate + H₂O 2,5-dioxopentanoate + NH₃

Thus, the two substrates of this enzyme are 1-pyrroline-4-hydroxy-2-carboxylate and H₂O, whereas its two products are 2,5-dioxopentanoate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is 1-pyrroline-4-hydroxy-2-carboxylate aminohydrolase (decyclizing). This enzyme is also called HPC deaminase. This enzyme participates in arginine and proline metabolism.

References

IUBMB entry for 3.5.4.22
BRENDA references for 3.5.4.22 (Recommended.)
PubMed references for 3.5.4.22
PubMed Central references for 3.5.4.22
Google Scholar references for 3.5.4.22
Singh RMM and Adams E (1965). "Enzymatic deamination of Delta1-pyrroline-4-hydroxy-2-carboxylate to 2,5-dioxovalerate (alpha-ketoglutaric semialdehyde)". J. Biol. Chem. 240: 4344–4351.
Singh RMM and Adams E (1965). "Isolation and identification of 2,5-dioxovalerate, an intermediate in the bacterial oxidation of hydroxyproline". J. Biol. Chem. 240: 4352–4356.